The PlayStation 3's Firmware 3.00 improves the XMB interface, and seems to help the Slim model load games and Blu-ray discs faster, but some users are reporting that their third-party controllers have stopped working since they installed the update.
The complaints are coming in via a big thread over at the official PlayStation 3 forum. Regardless of PS3 model: Some claim the issue affects their authentic DualShock 3, though it now looks like any problems may be specific to knock-off controllers.
Kotaku also mentions that a handful of users are reporting mid-game freezes in Uncharted: Drakes Fortune, but points out that it's not uncommon for this sort of thing to happen with PS3 or 360 system updates.
They've contacted Sony for comment, and we'll let you know what they have to say. In the meantime, have you run into any problems yourself? [Kotaku]